Node

NCBI Gene

Symbol mirn27b Gene ID  
IF 10.4% Link Number 12
Full Name MI0000440
Aliases miR-27b,hsa-mir-27b
Summary

miR-27 is a family of microRNA precursors found in animals, including humans. MicroRNAs are typically transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. The excised region or, mature product, of the miR-27 precursor is the microRNA mir-27. Herpesvirus saimiri expresses several non-coding RNAs (HSURs) which have been found to significantly reduce the level of mir-27 in a host cell. It has been proposed that miR-27 operates together with miR-23 and mir-24 in a co-operative cluster.

Node Summary

PMID: 22777896

As reaction to elevated plasma triglycerides (TGs) and liver steatosis, hepatic miR-27b was increased, and the ANGPTL3 and GPAM protein were repressed.As reaction to hypertriglyceridaemia and hepatic steatosis, hepatic miR-27b increases, leading to an inhibition of de novo biosynthesis of TGs.PPARα protein levels in human liver-derived cell lines were decreased by over-expression of miR-21 and miR-27b.

PMID: 19348006

Moreover, ectopic expression of miR-27a or miR-27b attenuates lipid accumulation.

PMID: 24133204

Computational prediction and luciferase assays have shown that miR-27a and miR-27b target prohibitin (PHB), which is highly expressed in cells that rely heavily on mitochondrial function and has recently been implicated in adipogenesis.

Node Expression

# PMID Condition Expression Material  
1 27562723 C2C12 adipogenic VS med VS myogenic (-1.5 2 -1) C2C12
2 23564456 BMP2 induced C2C12 osteo-dif(0 8) (1 0.4373) MC3T3-E1
3 20492721 MDI VS Li+MDI 3T3-L1 (1 2) (1 2.8) 3T3-L1
4 26531148 low VS high-abdominal fat group (1 2) (1 0.75) adipocyte
5 26001136 hMSC VS Adipocyte(1 2) (-2 2) MSC
6 24503542 Med-induced osteo-dif control vs exp (1 2) (0 1)  
7 21135128 preadipocytes VS adipocytes (1 2) (1 0.317) preadipocyte
8 20126310 Pre-adipocytes group(1 2) 1 -1.55 preadipocyte

Relation Chart

Chart Legend

Relation Table (12 records)

 
# Source IF(%) Relation Target IF(%) Type Method PMID
1 cold 2 inhibits mirn27b 10.4 others Bioinformatic,Cell culture and differentiation,Lipolysis assay,Northern blot,Overexpression and knockdown,Western blot 24238035
2 mirn27b 10.4 inhibits tgfb3 6.1 bind to RNA Microarray,Northern blot,Overexpression and knockdown,Reporter,RT-qPCR or RT-PCR,Western blot 19342382
3 mirn27b 10.4 inhibits ctnnbip1 2.5 bind to RNA Microarray,Northern blot,Overexpression and knockdown,Reporter,RT-qPCR or RT-PCR,Western blot 19342382
4 mirn27b 10.4 inhibits acvr2a 4.8 bind to RNA Microarray,Northern blot,Overexpression and knockdown,Reporter,RT-qPCR or RT-PCR,Western blot 19342382
5 mirn27b 10.4 inhibits dusp2 4.2 bind to RNA Microarray,Northern blot,Overexpression and knockdown,Reporter,RT-qPCR or RT-PCR,Western blot 19342382
6 mirn27b 10.4 inhibits lox 5.1 bind to RNA Overexpression and knockdown,Reporter,RT-qPCR or RT-PCR,Western blot 26536844
7 mirn27b 10.4 inhibits creb1 23.9 bind to RNA Bioinformatic,Cell culture and differentiation,Lipolysis assay,Northern blot,Overexpression and knockdown,Western blot 24238035
8 mirn27b 10.4 inhibits ppargc1a 16.3 bind to RNA Bioinformatic,Cell culture and differentiation,Lipolysis assay,Northern blot,Overexpression and knockdown,Western blot 24238035
9 mirn27b 10.4 inhibits phb 3.3 bind to RNA Cell culture and differentiation,Reporter,RT-qPCR or RT-PCR,Western blot 24133204
10 mirn27b 10.4 inhibits ppara 15 bind to RNA Bioinformatic,Cell culture and differentiation,Lipolysis assay,Northern blot,Overexpression and knockdown,Western blot 24238035
11 mirn27b 10.4 inhibits prdm16 7.7 bind to RNA Bioinformatic,Cell culture and differentiation,Lipolysis assay,Northern blot,Overexpression and knockdown,Western blot 24238035
12 mirn27b 10.4 inhibits apc 4.4 bind to RNA Cell culture and differentiation,Reporter,RT-qPCR or RT-PCR,Western blot 20708603

Prediction Chart

Chart Legend

Prediction Table (16 records)

 
# Source IF(%) Target IF(%) Species Agreement Computational Predictions / PMID
1 mirn27b 10.4 anxa5 4.6 Human -0.12 3
2 mirn27b 10.4 adora2b 1 Human 100.00 1
3 mirn27b 10.4 ddx17 2.7 Human 100.00 1
4 mirn27b 10.4 vdr 19.5 Human 100.00 1
5 mirn27b 10.4 pparg 77.1 Human 100.00 22120719
6 mirn27b 10.4 phgdh 6.4 Mouse -0.13 3
7 mirn27b 10.4 stom 5.2 Mouse -0.05 3
8 mirn27b 10.4 adora2b 1 Mouse 100.00 1
9 mirn27b 10.4 cyp1b1 7.1 Mouse 100.00 1
10 mirn27b 10.4 pparg 77.1 Mouse 100.00 1
11 mirn27b 10.4 smad3 13.4 Mouse 100.00 1
12 mirn27b 10.4 smad3 13.4 Mouse 100.00 1
13 mirn27b 10.4 jmjd1c 3.8 Mouse -0.03 3
14 mirn27b 10.4 dnm3 3.5 Mouse -0.02 3
15 mirn27b 10.4 mt-nd4 1 Mouse 0.02 3
16 mirn27b 10.4 diaph1 5 Human 100.00 1