Node

NCBI Gene

Symbol stat5a Gene ID 6776
IF 12.6% Link Number 11
Full Name signal transducer and activator of transcription 5A
Aliases MGF,STAT5
Summary

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Node Summary

PMID: 11110883

Signal transducers and activators of transcription (STATs) comprise a family of cytoplasmic proteins that are activated by and mediate gene expression in response to extracellular effectors that target receptors with intrinsic kinase activity or receptors to which Janus kinases (JAK) are bound.The receptor-bound STAT is phosphorylated,then dimerizes with other STAT proteins and translocates to the nucleus to mediate specific gene expression. The expression of three members of this family, STAT1, STAT5A and STAT5B, is upregulated during differentiation of cultured preadipocytes (Stephens et al. 1996).evidence indicates that the differentiation-dependent induction of STAT1, STAT5A and STAT5B is regulated downstream of PPARg in the differentiation paradigm (Fig. 2)(Stephens et al. 2000).

PMID: 17372307

STAT5A forms a complex with GR in the nucleus in the early phase of differentiation ,

PMID: 21427703

Using ChIP-seq analysis, we have recently shown that STAT5A and GR do in fact co-occupy multiple chromatin regions early in adipogenesis.

PMID: 17242167

Studies have shown that the GH-activated STAT5 proteins can induce PPARγ expression in 3T3-L1 cells and C3H10T1/2 cells

PMID: 21494231

Recent observations have shown that ectopic STAT5A expression can confer the adipogenic capabilities of Swiss 3T3 fibroblasts in athymic mice

Node Expression

# PMID Condition Expression Material  
1 27385551 human preadipocyte dif (0 192) hours (0 3.91757) preadipocyte
2 27385551 human preadipocyte dif (0 384) hours (0 3.90684) preadipocyte
3 27385551 human preadipocyte dif (0 96) hours (0 3.49357) preadipocyte
4 27385551 human preadipocyte dif (0 48) hours (0 2.26429) preadipocyte
5 27385551 human preadipocyte dif (6 48) hours (0 1.69001) preadipocyte
6 27385551 human preadipocyte dif (48 96) hours (0 1.22928) preadipocyte
7 27108396 Control VS hADSC day 21 post-adipogenic induction (1 2.17) hADSC
8 27108396 Control VS hADSC day 14 post-adipogenic induction (1 1.85) hADSC
9 27108396 Control VS hADSC day 7 post-adipogenic induction (1 1.39) hADSC
10 26111340 hMSC High Density & DMI-Induced High expression Up-Regulated MSC
11 26111340 hMSC DMI-Induced High expression Up-Regulated MSC
12 12021175 Ratio(1 2 3 4 5)preadi/fibro,TZD-preadi/preadi,adi/fibro,adi/preadi,TZD-adi/adi ratio(0 0 0 1 0） 3T3-L1

Relation Chart

Chart Legend

Relation Table (11 records)

 
# Source IF(%) Relation Target IF(%) Type Method PMID
1 fndc3b 4 inhibits stat5a 12.6 phosphorylation Cell culture and differentiation,Histology,Immunofluorescence,(IP)immunoprecipitation,Overexpression and knockdown,RT-qPCR or RT-PCR,Western blot 24052261
2 gh1 4.1 activates stat5a 12.6 others EMSA 8732682
3 gr-stat5a 0.5 activates stat5a 12.6 others   17372307
4 jak2 8.6 activates stat5a 12.6 others (IP)immunoprecipitation,Cell culture and differentiation,Western blot 10085104
5 pparg 77.1 activates stat5a 12.6 others Cell culture and differentiation,Overexpression and knockdown,RT-qPCR or RT-PCR,Western blot 10448095
6 socs2 7.4 inhibits stat5a 12.6 signal transduction Overexpression and knockdown,RT-qPCR or RT-PCR,Triacylglycerol (TAG) Measurement,Western blot 22729878
7 stat 0.8 activates stat5a 12.6 others   00000001
8 stat5a 12.6 inhibits bcl6 12.9 bind to DNA Bioinformatic,ChIP,Reporter,Western blot 19406940
9 stat5a 12.6 activates igf1 20.1 others Bioinformatic,ChIP,Reporter,Western blot 19406940
10 stat5a 12.6 activates socs2 7.4 others Bioinformatic,ChIP,Reporter,Western blot 19406940
11 stat5a 12.6 activates pparg 77.1 others (IP)immunoprecipitation,Cell culture and differentiation,RT-qPCR or RT-PCR,Western blot 12089351

Prediction Chart

Chart Legend

Prediction Table (15 records)

 
# Source IF(%) Target IF(%) Species Agreement Computational Predictions / PMID
1 esr1 17.1 stat5a 12.6 Human 100.00 11682624
2 mirn222 12.6 stat5a 12.6 Human 100.00 1
3 ppara 15 stat5a 12.6 Human 100.00 21542777
4 ppara 15 stat5a 12.6 Human 100.00 21542777
5 ppara 15 stat5a 12.6 Human 100.00 21542777
6 stat5a 12.6 bcl6 12.9 Human 100.00 16819511
7 stat5a 12.6 bcl6 12.9 Human 100.00 16819511
8 stat5a 12.6 ccnd1 26.2 Human 100.00 14645506
9 stat5a 12.6 ccnd2 13.3 Human 100.00 22723377
10 stat5a 12.6 cdkn2b 7.5 Human 100.00 22723377
11 stat5a 12.6 cel 0.9 Human 100.00 9841868
12 stat5a 12.6 esr1 17.1 Human 100.00 11731618
13 stat5a 12.6 ifng 5.6 Human 100.00 15528362
14 stat5a 12.6 il10 6.4 Human 100.00 23797738
15 stat5a 12.6 sumo1 2.7 Human 100.00 22723377