NCBI Gene
| Symbol | ube2i | Gene ID | 7329 |
| IF | 2.7% | Link Number | 1 |
| Full Name | ubiquitin-conjugating enzyme E2I | ||
| Aliases | C358B7.1,P18,UBC9 | ||
| Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
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Node Summary
PMID: 26192107
we performed a high-throughput microscopy screen that identified ubiquitin carrier protein 9 (Ubc9) as a negative regulator of energy storage in human sc adipocytes. Ubc9 depletion enhanced energy storage and induced the brown fat gene program in human sc adipocytes. Induction of adipocyte differentiation resulted in decreased Ubc9 expression commensurate with increased brown fat gene expression. Thiazolidinedione treatment reduced the interaction between Ubc9 and peroxisome proliferator-activated receptor (PPAR)γ, suggesting a mechanism by which Ubc9 represses PPARγ activity. In support of this hypothesis, Ubc9 overexpression remodeled energy metabolism in human sc adipocytes by selectively inhibiting brown adipocyte-specific function. Further, Ubc9 overexpression decreased uncoupling protein 1 expression by disrupting PPARγ binding at a critical uncoupling protein 1 enhancer region. Last, Ubc9 is significantly elevated in sc adipose tissue isolated from mouse models of insulin resistance as well as diabetic and insulin-resistant humans.
Node Expression
| # | PMID | Condition | Expression | Material | |
|---|---|---|---|---|---|
| 1 | 19300518 | PPARg-RXR target | fold(7)/IgG | 3T3-L1 |
Relation Chart
Chart Legend
Relation Table (1 record)
| # | Source | IF(%) | Relation | Target | IF(%) | Type | Method | PMID |
|---|---|---|---|---|---|---|---|---|
| 1 | ube2i | 2.7 | inhibits | pparg | 77.1 | bind to protein | Cell culture and differentiation,Overexpression and knockdown,RT-qPCR or RT-PCR | 26192107 |
Prediction Chart
Chart Legend
Prediction Table (14 records)
| # | Source | IF(%) | Target | IF(%) | Species | Agreement | Computational Predictions / PMID |
|---|---|---|---|---|---|---|---|
| 1 | mirn188 | 3 | ube2i | 2.7 | Human | 100.00 | 1 |
| 2 | mirn200c | 7.7 | ube2i | 2.7 | Human | 100.00 | 1 |
| 3 | mirn30a | 17.6 | ube2i | 2.7 | Human | 100.00 | 1 |
| 4 | mirn30a | 17.6 | ube2i | 2.7 | Mouse | 100.00 | 1 |
| 5 | mirn30b | 13.9 | ube2i | 2.7 | Mouse | 100.00 | 1 |
| 6 | mirn30b | 13.9 | ube2i | 2.7 | Human | 100.00 | 1 |
| 7 | mirn30c | 14.4 | ube2i | 2.7 | Human | 100.00 | 2 |
| 8 | mirn30c | 14.4 | ube2i | 2.7 | Mouse | 100.00 | 1 |
| 9 | mirn30d | 8.8 | ube2i | 2.7 | Mouse | 100.00 | 1 |
| 10 | mirn30d | 8.8 | ube2i | 2.7 | Human | 100.00 | 1 |
| 11 | mirn30e | 16.7 | ube2i | 2.7 | Human | 100.00 | 3 |
| 12 | mirn30e | 16.7 | ube2i | 2.7 | Mouse | 100.00 | 1 |
| 13 | mirn324 | 10.2 | ube2i | 2.7 | Human | 100.00 | 1 |
| 14 | mitf | 6.1 | ube2i | 2.7 | Human | 100.00 | 10694430 |
